Microscopy
Method overview
Place a thin specimen on a slide, add an appropriate stain, lower a coverslip at an angle, begin with the lowest-power objective, focus, then increase magnification. Produce a clear biological drawing with labels and a scale or magnification.
Apparatus set-up
Diagramlight microscope; slide; coverslip; specimen; stain; forceps; mounted needle
Independent variable
specimen or magnification used
Dependent variable
visible cell structures and measured image size
Control variables
same microscope calibration; same staining approach when comparing
Apparatus
light microscope; slide; coverslip; specimen; stain; forceps; mounted needle
Safety
Handle glass slides and stains carefully; use forceps or a mounted needle rather than fingers.
Scientific rationale
A thin specimen lets light pass through; stain increases contrast; lowering the coverslip at an angle reduces trapped air bubbles.