biologyRP3 · Cell BiologyPaper 1Combined and Separate

Osmosis in plant tissue

Method overview

Cut equal potato cylinders, measure initial mass, place each in a different known concentration for the same time, remove, blot in a standard way and reweigh. Calculate percentage mass change and plot against concentration.

Apparatus set-up

Diagram
Potato cylinders in solution
0.0
Mass before / after
Length before / after

potato cylinders; cork borer; ruler; balance; labelled tubes; sugar/salt solutions; paper towel; timer

Independent variable

solution concentration

Dependent variable

change or percentage change in tissue mass

Control variables

initial cylinder dimensions; tissue source; solution volume; time; temperature; blotting method

Apparatus

potato cylinders; cork borer; ruler; balance; labelled tubes; sugar/salt solutions; paper towel; timer

Safety

Cut away from fingers and handle glassware/solutions carefully.

Scientific rationale

Water moves through partially permeable cell membranes from higher water concentration to lower water concentration. Blotting removes surface liquid so only tissue mass is measured.

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