Osmosis in plant tissue
Method overview
Cut equal potato cylinders, measure initial mass, place each in a different known concentration for the same time, remove, blot in a standard way and reweigh. Calculate percentage mass change and plot against concentration.
Apparatus set-up
Diagrampotato cylinders; cork borer; ruler; balance; labelled tubes; sugar/salt solutions; paper towel; timer
Independent variable
solution concentration
Dependent variable
change or percentage change in tissue mass
Control variables
initial cylinder dimensions; tissue source; solution volume; time; temperature; blotting method
Apparatus
potato cylinders; cork borer; ruler; balance; labelled tubes; sugar/salt solutions; paper towel; timer
Safety
Cut away from fingers and handle glassware/solutions carefully.
Scientific rationale
Water moves through partially permeable cell membranes from higher water concentration to lower water concentration. Blotting removes surface liquid so only tissue mass is measured.